金亚

 

基本信息

单位：广东工业大学 医药生物学院

职称：教授（硕导） 学位：博士 出生日期：1975年

研究领域：蛋白质组学、生物质谱、分子病理药理

Email：yajin@gdut.edu.cn

 

教育经历

1997.09－2002.07：清华大学化学系，分析化学专业，获博士学位

1992.09－1997.07：清华大学化学系，物理化学及仪器分析专业，获学士学位

 

工作经历

2017.3至今：广东工业大学，生物医药研究院，教授

2012.10－2017.02：华南理工大学，生物科学与工程学院，副研究员/副教授

2005.04－2012.04：日本爱媛大学，理工学研究科，外国人研究员

2003.04－2005.03：日本爱媛大学理学部，日本学术振兴会（JSPS）外国人特别研究员

 

承担项目

在研主持项目：广东工业大学引进人才项目1；已结题主持项目：国家自然科学基金青年科学基金项目1、华南理工大学中央高校基本科研业务费重点项目1、广州市科技计划项目1、广东省发酵与酶工程重点实验室开放基金项目2、华南理工大学中央高校基本科研业务费面上项目1。

 

教学活动

已毕业硕士研究生6名，现指导博士研究生1名，硕士研究生1名。

主讲本科生必修课《生物化学及分子生物学》、《现代生化技术》；硕士研究生必修课《SCI文章写作与优秀外文科技文章导读》，硕士及博士研究生选修课《蛋白质组学分析技术及蛋白质组信息学》、《专业英语》等。

 

代表性著作及论文

申请人多年从事蛋白质组学的研究，系统地建立了非变性蛋白质组学的研究平台，其中涉及到多项针对复杂蛋白质体系的非变性二维/多维凝胶电泳及质谱分析方法的建立，旨在获得蛋白质及蛋白质复合物在生理条件下的结构、功能及相互作用的信息。已发表英文著作1章，论文40篇（SCI论文28篇）。

 

著作：

Takashi Manabe, Ya Jin, in: Hughes AB (Ed.), Amino acids, Peptides and Proteins in Organic Chemistry: Volume 5: Analysis and Function of Amino Acids and Peptides, Wiley-VCH Verlag GmbH & Co. KGaA , Weinheim 2011, Chapter 13 “Two-dimensional gel electrophoresis and protein/polypeptide assignment”, pp. 439-462.  

 

论文：

1. Meiling Wen, Ya Jin*, Hao Zhang, Xiaoou Sun, Yihe Kuai, Wen Tan*, Proteomic Analysis of Rat Cerebral Cortex in the Sub-Acute to Long-Term Phases of Focal Cerebral Ischemia-Reperfusion Injury. J. Proteome Res. 2019, online (DOI: 10.1021/acs.jproteome.9b00220).

2. Ya Jin*, Meiling Wen, Qi Yuan, Jun Zhang, Wen Tan*, Beneficial effects of Coomassie staining on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS. J. Chromatogr. B 2019, 1110-1111, 25-35.

3. Ya Jin*, Jun Zhang, Takashi Manabe, Wen Tan*, Comparison of the performance of 1D SDS-PAGE with nondenaturing 2DE on the analysis of proteins from human bronchial smooth muscle cells using quantitative LC-MS/MS. J. Chromatogr. B 2019, 1105, 193-202.

4. Meiling Wen, Ya Jin*, Takashi Manabe, Shumin Chen, Wen Tan*, A comparative analysis of human plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS: Utilizing native MS-electropherograms in proteomic analysis for discovering structure and interaction-correlated differences. Electrophoresis 2017, 38(24), 3111–3123.

5. Shumin Chen, Meiling Wen, Shujie Bu, Ahui Wang, Ya Jin*, Wen Tan*. Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and quantitative LC-MS/MS. Electrophoresis 2016, 37(23-24), 3126-3136.

6. Lixian Cheng, Hui Shi, Yan Jin, Xiaoxi Li, Jinshun Pan, Yimei Lai, Yan Lin, Ya Jin, Gaurab Roy, Allan Zhao, Fanghong Li. Adiponectin Deficiency Leads to Female Subfertility and Ovarian Dysfunctions in Mice. Endocrinology 2016, 157(12), 4875-4887.

7. Ahui Wang, Xiaoou Sun, Qing Liu, Tingting Hu, Ting Han, Ya Jin*, Wen Tan*. The protective effects of Sildenafil on transverse aortic constriction induced cardiac hypertrophy in rats. Journal of Guangdong College of Pharmacy (China) 2016, 32(4), 475-480.

8. Jie Wu, Yiguang Tian, Shanping Wang, Marco Pistolozzi, Ya Jin, Ting Zhou, Gaurab Roy, Ling Xu, Wen Tan. Design, synthesis and biological evaluation of bambuterol analogues as novel inhibitors of butyrylcholinesterase. Eur J Med Chem. 2016,126, 61-71.

9. Ya Jin, Jin Chen, Ahui Wang, Jun Zhang, Shumin Chen, Takashi Manabe, Wen Tan*. Analysis of low-density lipoprotein-associated proteins using the method of digitized native protein mapping. Electrophoresis 2016, 37(14), 2063-2074.

10. Ya Jin, Qi Yuan, Jun Zhang, Takashi Manabe, Wen Tan. Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 2. Similarity search between protein maps for the analysis of protein complexes. Electrophoresis 2015, 36(17), 1991-2001.

11. Ya Jin, Jun Zhang, Qi Yuan, Takashi Manabe, Wen Tan. Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 1. Preparation of more than 4000 native protein maps. Electrophoresis 2015, 36(15), 1711-1723.

12. Jun Zhang, Ya Jin*, Yanrui Ye, Wen Tan. Technologies in G-protein-coupled receptor-ligand binding assays. Chinese Journal of Pharmaceutical Analysis 2015, 35(1), 1-7.

13. Ya Jin, Shujie Bu, Jun Zhang, Qi Yuan, Takashi Manabe, Wen Tan. Native protein mapping and visualization of protein interactions in the area of human plasma high-density lipoprotein by combining nondenaturing micro 2DE and quantitative LC-MS/MS. Electrophoresis 2014, 35(14), 2055-2064.

14. Takashi Manabe, Ya Jin. Performance of non-denaturing micro 2-DE followed by third-dimension SDS-PAGE in the analysis of Escherichia coli soluble proteins. Electrophoresis 2011, 32(2), 300-309.

15. Takashi Manabe, Ya Jin. Analysis of E. coli soluble proteins by non-denaturing micro 2-DE/3-DE and MALDI-MS-PMF. Electrophoresis 2010, 31(16), 2740-2748.

16. Takashi Manabe, Ya Jin. Assignment of human plasma proteins on non-denaturing micro 2-DE gels using MALDI-MS. Seibutsu Butsuri Kagaku 2010, 54(1), 31-34. Japanese.

17. Ya Jin, Takashi Manabe. Analysis of polyethylene glycol-fractionated high-molecular-mass proteins in human plasma by non-denaturing micro 2-DE and MALDI-MS PMF. Electrophoresis 2009; 30(20), 3613-3621.

18. Ya Jin, Takashi Manabe. Differences in protein distribution between human plasma preparations, EDTA-plasma and heparin-plasma, analyzed by non-denaturing micro 2-DE and MALDI-MS PMF. Electrophoresis 2009; 30(6), 931-938.

19. Ya Jin, Takashi Manabe. Performance of agarose IEF gels as the first dimension support for non-denaturing micro 2-DE in the separation of high-molecular-mass plasma proteins and protein complexes. Electrophoresis 2009; 30(6), 939-948.

20. Takashi Manabe, Ya Jin. Noncovalent interactions in human plasma proteins analyzed by the comparison of nondenaturing and denaturing micro-2-D gel electrophoresis patterns after polypeptide assignment using matrix-assisted laser desorption/ionization-mass spectrometry and peptide mass fingerprinting. Electrophoresis 2008; 29(12), 2672-2688.

21. Takashi Manabe, Ya Jin, Nao Yamaguchi, Tetsuo Sugiyama, Kohei Ikari. Cleavage of fibrinogen alpha chains during isoelectric focusing of human plasma under non-denaturing conditions analyzed by micro two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry. Journal of Electrophoresis 2007; 51(3/4), 27-34.

22. Takashi Manabe, Ya Jin. Analysis of protein/polypeptide interactions in human plasma using nondenaturing micro-2-DE followed by 3-D SDS-PAGE and MS. Electrophoresis 2007; 28(12), 2065-2079.

23. Takashi Manabe, Ya Jin, Osamu Tani. Assignment of human plasma polypeptides on a nondenaturing 2-D gel using MALDI-MS and PMF and comparisons with the results of intact protein mapping. Electrophoresis 2007; 28(5), 843-863.

24. Ya Jin, Takashi Manabe. Alkaline extraction of human plasma proteins from nondenaturing micro-2-D gels for protein/polypeptide mass measurement and peptide mass fingerprinting using MALDI-TOF MS. Electrophoresis 2007; 28(3), 449-459.

25. Maki Kamakura, Keiko Morisawa, Hideyuki Komi, Ayako Tomatani, Fumiji Saito, Yuko Konishi, Ya Jin, Takashi Manabe, Masayuki Kuroda, Shosuke Imai, Hiroyuki Mizuguchi, Taketoshi Taniguchi. Regulation of IL-27p28 gene by lipopolysaccharide in dendritic DC2.4 cells. Biochemical and Biophysical Research Communications 2006; 349(4), 1372-1377.

26. Ya Jin, Takashi Manabe. Direct targeting of human plasma for matrix-assisted laser desorption/ionization and analysis of plasma proteins by time of flight-mass spectrometry. Electrophoresis 2005; 26(14), 2823-2834.

27. Ya Jin, Takashi Manabe. High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis 2005; 26(6), 1019-1028.

28. Takashi Manabe, Ya Jin. Alkaline cleavage of covalent bonds in chicken insulin and bovine -lactalbumin analyzed by matrix-assisted laser desorption/ionization-mass spectrometry. Electrophoresis 2005; 26(1), 257-267.

29. Ya Jin, Guo-an Luo. Numerical calculation of the electroosmotic flow at the cross region in microfluidic chips. Electrophoresis 2003; 24(7-8), 1242-1252.

30. Ya Jin, Guo-an Luo. Fabrication of the microfluidic chips with integrated ultra-micro electrodes and its application in on-chip electrochemical detection. Chemical Journal of Chinese Universities 2003, 24(7), 1180-1184. Chinese.

31. Ya Jin, Guoan Luo, Tomoko Oka, Takashi Manabe. Estimation of isoelectric points of human plasma proteins employing capillary isoelectric focusing and peptide isoelectric point markers. Electrophoresis 2002; 23(19), 3385-3391.

32. Ya Jin, Guo-an Luo. Fabrication and applications of an interface to couple microfluidic chips with electrospray ionization mass spectrometer. Analytical Instrumentation (China) 2003, (2), 4-10.

33. Guang-hua Zhang, Zhao-ying Zhou, Yang-hua Tang, Guo-an Luo, Ya Jin. The development of micro chip electrophoresis analyser. Chinese Journal of Scientific Instrument 2002, (4), 335-338.

34. Guang-hua Zhang, Zhao-ying Zhou, Yang-hua Tang, Guo-an Luo, Ya Jin. Design of a biochemical substance analysis system. Measurement & Control Technology (China) 2002, 21(4), 10-12.

35. Yang-hua Tang, Xiong-ying Ye, Zhao-ying, Zhou, Ya Jin, Yan-ying Feng. Glass chips for capillary electrophoresis, micromachining, sample injection and separation. Chinese Journal of Analytical Chemistry 2001, 29(5), 606-610.

36. Ya Jin, Tao Wen, Yi-ming Wang, Guo-an Luo. Development and application of the detection technologies of integrated capillary electrophoresis chips. Modern Scientific Instruments (China) 2001, (4), 15-17.

37. Yang-hua Tang, Zhao-ying Zhou, Xiong-ying Ye, Yan-ying Feng, Ya Jin. Microfabrication of capillary electrophoresis chips. Microfabrication Technology (China) 2001, (1), 53-55.

38. Ya Jin, Guo-an Luo, Yang-hua Tang, Zhao-ying Zhou. Microfabrication of integrated capillary electrophoresis chips and on-chip sample injection and separation. Journal of Analytical Science (China) 2001, 17(2), 148-152.

39. Ya Jin, Guo-an Luo. Development of technology and applications of microarrays. Acta Pharmaceutica Sinica (China) 2000, 35(3), 235-240.

40. Ya Jin, Guo-an Luo, Ru-ji Wang. Development of integrated capillary electrophoresis chips. Se Pu (China) 2000, 18(4), 313-317.